During the heavy rain more than 32,000 gallons of sewage spilled from a location on North School Street.
The health department says the spill started Wednesday night around 10:40 p.m.
It lasted about four hours enough to release 32,400 gallons of wastewater.
Crews were able to recover about 800 gallons but more than 31,000 gallons entered a storm drain, which empties into the Kapalama Canal and eventually Honolulu Harbor.
The area was cleaned and disinfected.